I vores artikelarkiv kan du dykke ned i nogle af de nyeste forskningsresultater og laboratorieerfaringer. Lær hvordan automatisering og innovative teknologier som f.eks. CyBio FeliX kan forbedre din arbejdsproces og øge produktiviteten i dit laboratorium.
Find mere om:
- Celletransformation og automatisering
- Effektiv plasmid-DNA-isolation
- Optimering af cellekulturer
- Vores partnersamarbejde med SynbiCITE og Analytik Jena
- Ekspertise i laboratorieapplikationer
- Videnskabelige artikler og ressourcer
Automated High-Throughput Transformation of Bacteria Cells
The transformation of bacteria cells typically remains a manual, low-throughput activity in molecular biology laboratories. The ability to transform bacteria
transformation in a high-throughput, automated manner would greatly aid laboratories looking to increase their throughput and improve the robustness and reproducibility of their experiments.
Automated High-Throughput Plasmid DNA Isolation from Bacteria Cells
Plasmid DNA isolation is an essential molecular biology technique, however, highthroughput automation of the method has proved challenging. SynbiCITE and
Analytik Jena have developed a method using the CyBio FeliX pipetting platform for the isolation of high quality plasmid DNA from 96 samples simultaneously,
yielding concentrations appropriate for downstream applications such as sequencing.
Centrifuge-Free Clarification of Large-Volume Bacterial Cultures Using Sartoclear Dynamics® Lab
Clarification of bacterial cultures is often performed by centrifugation and subsequent dead-end microfiltration, a cumbersome and time-consuming process, particularly when large volumes of cultures need to be treated. One example is the production of vaccine prototypes based on outer membrane vesicles (OMVs), a type of bacterial vesicle which has been shown to possess great immunogenic potential and has been a central focus of the research conducted in our group.
Concentration of Mammalian Cell Culture Supernatants with Vivaspin® Turbo 15 PES and Amicon® Ultra-15 Devices
Mammalian cell culture molecule separation processes commonly rely on concentration methods to provide molecule of interest concentration values, that are needed for various downstream processes, such as western blot and mass spectrometry analysis. Ultrafiltration techniques enable rapid and precise concentration across a range of volumes, but can incur loss of yield if sub-optimal membrane, molecular weight cut off (MWCO) or device protocols are employed. Here we benchmark two different membranes and MWCO devices for the concentration of a molecule of interest: Trefoil factor 1, to demonstrate the resulting differences in yield percentage recovery.
From Mammalian Cell Cultures to Pure Proteins: Sartoclear Dynamics® Lab Significantly Reduces Cell Harvest Time
In this study, the novel Sartoclear Dynamics® Lab V Kit was evaluated for the removal of transiently IgG expressing mammalian MEXi-293E (HEK293) cells from cell cultures. The method was directly compared to the present standard method that required two centrifugation steps. After clarification, recombinant IgG harboring a Twin-Strep-tag® was purified from all samples in parallel by a one-step Strep-Tactin®XT Superflow® high capacity affinity purification process.
Lab-Scale Clarification of Mammalian Suspension Cultures Using Sartoclear Dynamics® Lab V Kits
This study evaluates a novel method for lab-scale clarification of mammalian cell culture supernatants. The filtration step is conducted prior to purification of monoclonal antibodies with protein A affinity chromatography. In a conventional clarification process, cells are removed by a time-consuming centrifugation step followed by filtration. We show that the Sartoclear Dynamics® Lab V Kit is not only a significant time-saving method compared with the conventional clarification process. It also yields an improved clearance rate that we observed in combination with high flow rate filtration. For clarification of large-volume cell cultures, Sartoclear Dynamics® Lab V Kits are an attractive addition to standard lab instrumentation to increase productivity and throughput prior to protein purification.
Minimizing Syringe Filter Usage in Harvesting Monoclonal Antibodies from CHO Cell Culture Supernatants
Clarification of cell culture supernatants with volumes of < 25 mL for harvesting monoclonal antibodies by using syringe filters is often a laborious and, sometimes, an exhausting step. Therefore, selection of a suitable filter type is essential. In this study, we compared the performance characteristics of two suppliers’ syringe filter types, each with a similar effective filtration area, for clarification of CHO cell culture supernatant samples.
0.1-μm Bottle-Top Filtration of Media as a Strategy to Avoid Contamination of Cell Cultures with Mycoplasma
Preventing contamination with mycoplasma plays a crucial role when working with cell cultures in order to ensure accurate and reproducible results in experimental trials and production of biological targets. To minimize the risk of contamination, it is recommended to implement a number of measures, such as aseptic working, use of mycoplasma-free tested reagents and media, and regular mycoplasma testing.
Rapid Mammalian Cell Harvest without Centrifugation for Antibody Purification Using the Sartoclear Dynamics® Lab Filtration System
Monoclonal antibody expression systems typically utilise a signal peptide to ensure secretion of the antibody into the cell culture media. Although this reduces the complexity of purification and avoids the need for cell disruption, it does require the use of expensive and/or time-consuming techniques to separate cells from antibody-containing cell culture fluid. In this study, we describe our tests of Sartoclear Dynamics® Lab V, a novel system for rapid clarification of cell culture media without the need for centrifugation or any other costly equipment.
Reducing sample preparation time from Sf9 insect cultures by using Sartoclear Dynamics® Lab
In this study, I tested the Sartoclear Dynamics® Lab V500 Kit as a novel method for the clarification of cell culture media prior to purification of a recombinant protein expressed in Sf9 cells. The vector from which this protein is expressed encodes a signal sequence to ensure secretion of the protein from the cells following expression. It is therefore necessary to remove cells from the media before purifying the protein but using traditional methods to do this (centrifugation followed by filtration) can be time consuming.
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